{"created":"2024-06-25T04:32:53.633792+00:00","id":2000125,"links":{},"metadata":{"_buckets":{"deposit":"c0f36bef-3350-4bba-bdfc-9259c0fc65ab"},"_deposit":{"created_by":11,"id":"2000125","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000125"},"status":"published"},"_oai":{"id":"oai:kinjo.repo.nii.ac.jp:02000125","sets":["113","113:18","113:18:1719192528703"]},"author_link":[],"control_number":"2000125","item_10002_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"27","bibliographicPageStart":"18","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"金城学院大学論集. 自然科学編","bibliographic_titleLang":"ja"},{"bibliographic_title":"Treatises and Studies by the Faculty of Kinjo Gakuin University. Studies in Natural Sciences","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"腸内細菌が産生する有機酸・短鎖脂肪酸は，腸管免疫に重要な役割を果たすことが知られている。しかし，有機酸・短鎖脂肪酸の抗アレルギー効果についてはあまり報告されていない。本研究では，有機酸・短鎖脂肪酸が体内に取り込まれ，マスト細胞に作用することで，アレルギーを抑制するのではないかとの仮説を立て，ラット好塩基球様細胞株RBL-2H3細胞を用いて，有機酸・短鎖脂肪酸の脱顆粒抑制効果および細胞内Ca2＋濃度に及ぼす影響について検討した。9 種類の有機酸・短鎖脂肪酸を用いて，RBL-2H3細胞への細胞毒性を評価した後，各種成分刺激による抗アレルギーの評価として脱顆粒抑制試験，脱顆粒抑制メカニズムの評価として細胞内Ca2＋濃度の測定を行った。脱顆粒抑制試験は，有機酸・短鎖脂肪酸の種類により，脱顆粒抑制効果に差が認められたため，脱顆粒抑制効果が強かった 6 サンプルに絞り，同濃度で比較した結果，コハク酸＞酢酸＞イソ酪酸＝イソ吉草酸＞酪酸＝吉草酸の順に脱顆粒抑制効果が強いことが明らかとなった。β-hexosaminidase放出率と細胞内Ca2＋濃度の相関について検討した結果，非常に強い正の相関が見られた（p＜0.05, R＝0.97）。以上の結果から，有機酸・短鎖脂肪酸には抗アレルギー効果が認められ，有機酸・短鎖脂肪酸の抗アレルギー効果にはCa2＋濃度が関係していることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"identifier_registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.69275/0002000125","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"金城学院大学","subitem_publisher_language":"ja"}]},"item_10002_source_id_9":{"attribute_name":"item_10002_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"18800378","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"横山, さや香","creatorNameLang":"ja"},{"creatorName":"YOKOYAMA, Sayaka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"三宅, 香穂","creatorNameLang":"ja"},{"creatorName":"MIYAKE, Kaho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"田中, 守","creatorNameLang":"ja"},{"creatorName":"TANAKA, Mamoru","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-07-08"}],"filename":"03_Sayaka YOKOYAMA, Kaho MIYAKE, Mamoru TANAKA.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kinjo.repo.nii.ac.jp/record/2000125/files/03_Sayaka YOKOYAMA, Kaho MIYAKE, Mamoru TANAKA.pdf"},"version_id":"90b7f95e-dcf6-4f44-aeae-e581e6bbe678"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ラット好塩基球様細胞株RBL-2H3の脱顆粒に及ぼす 有機酸・短鎖脂肪酸の影響と細胞内Ca2＋濃度の関係","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ラット好塩基球様細胞株RBL-2H3の脱顆粒に及ぼす 有機酸・短鎖脂肪酸の影響と細胞内Ca2＋濃度の関係","subitem_title_language":"ja"},{"subitem_title":"Relationship between the Antiallergic Effects of Organic Acids and Short-Chain Fatty Acids and Intracellular Ca2＋ Concentration in Rat Basophilic Leukemia Cell Line RBL-2H3","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["113","18","1719192528703"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-08"},"publish_date":"2024-07-08","publish_status":"0","recid":"2000125","relation_version_is_last":true,"title":["ラット好塩基球様細胞株RBL-2H3の脱顆粒に及ぼす 有機酸・短鎖脂肪酸の影響と細胞内Ca2＋濃度の関係"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-02-10T05:04:25.477344+00:00"}